Trouble is a brewin’ at this watering hole.

A Massachusetts lesbian bar descended into a catty culture war and faces a potentially business-ending boycott from sudden enemies who flipped out when the owners lifted a longstanding mask mandate — for just one night a week.

Greenfield’s Last Ditch Bar scrapped Saturday mask requirements on Aug. 31 in a bid to boost business — but the move completely backfired, sparking uproar from members of the small city’s queer community.

Some critics dramatically likened the decision to a “mass disabling event,” a “eugenicist” attack and a host of other hot-button terms that escalated to threats of violence.

“I…

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